Yang Shuhua

Yang Shuhua

Yang Shuhua, female, doctor, Professor, Department of plant science, China Agricultural University, distinguished professor, Changjiang Scholar.

Character experience

Educational experience

From September 1987 to June 1991, Bachelor of science, Nankai University; from September 1991 to June 1994, master of science, Nankai University; from July 1998 to January 2002, doctor of science, Department of Biological Sciences, National University of Singapore

work experience

From July 1994 to June 1998, research intern and assistant researcher, Institute of elemental organic chemistry, Nankai University; from February 2002 to December 2004, postdoctoral, Department of plant science, Cornell University; from January 2005 to present, Professor, School of biology, China Agricultural University

Research direction

Molecular mechanism of plant response to low temperature stress as the main environmental factor, temperature affects the growth and development of plants, the distribution of vegetation and the yield of crops. Our laboratory mainly studies the signal transduction pathway of plant response to low temperature stress, the interaction between low temperature signal and plant hormone signal, and the interaction between low temperature signal and biological stress signal by means of molecular biology, genetics and biochemistry.

one

Molecular regulation of plant response to sub zero low temperature stress

in the research of plant freezing resistance, we have established a stable screening system of freezing related mutants. Through this system, we found that protein kinase crpk1 (cold responsive protein kinase 1) is involved in CBF dependent signal transduction, which negatively regulates the key transcription factor of cold signaling pathway. Low temperature activates crpk1 located on the cell membrane, which phosphorylates 14-3-3 protein in the cytoplasm, induces it to enter the nucleus, interacts with CBF1 / 3 protein, and mediates ubiquitination degradation of CBF1 / 3 protein, thus negatively regulating plant response to low temperature. This study not only revealed the negative regulation mechanism of cold signal transmission from cell membrane to nucleus, but also elucidated the regulation mechanism of CBF protein post-translational modification (Liu et al., 2017, molcell). Protein kinase OST1 plays an important and positive role in plant cold response. The activity of OST1 kinase is rapidly induced by low temperature. Activated OST1 phosphorylates ICE1 and inhibits the ubiquitination of ICE1, thus regulating the freezing resistance of plants (dinget al., 2015, devcell). in the cross interaction of low temperature signal and plant hormone signal, we found that the key components of ethylene signaling pathway are involved in low temperature response. The transcription factor EIN3 protein directly and negatively regulates the expression of CBF and arr genes, thus negatively regulating the freezing resistance of Arabidopsis thaliana. This study not only clarified the role of ethylene in plant cold resistance, but also revealed the molecular mechanism of the interaction between ethylene and cytokinin signaling (Shi et al., 2012, plantcell). BIN2 and bzr1 / bes1, the key protein kinase of brassinolide signaling pathway, regulate the expression of a series of downstream genes in a CBF dependent and CBF independent manner, thus positively regulating the freezing resistance of plants (lietal., 2017, molplant). Using CRISPR technology, we constructed three deletion mutants of CBFS, identified the key role of CBFS in low temperature acclimation and target genes of CBFS, and provided ideal genetic materials for low temperature signal research (jiaet al., 2016, newphytol).

two

Molecular regulation of plant response to above zero low temperature stress

our study found that resistance related genes CHS1, rpp4 / Chs2, chs3 and LSD1 / cHS4, which play an important role in defense response of Arabidopsis thaliana, participate in the regulation of plant growth homeostasis under different temperatures (wangetal., 2013, plantj; wangetal., 2011, plantj; Huang et al., 2010, plantphysical; yangetal., 2010, plantj; Huang et al., 2010, newPhysical). Studies have revealed that tir-nb protein CHS1 interacts with R protein soc3, protein phosphatase ibr5 and chaperone Hsp90 interact with R protein chs3 and Chs2, respectively, to regulate R protein activity, thereby regulating R-mediated temperature-dependent cell death and defense response (Zhang et al., 2016, newphytol; Liu et al., 2015, plosgenet; Bao et al., 2014, newphytol).
Studies have found that Arabidopsis phospholipid binding protein bon1 is a key regulator in the regulation of temperature dependent growth and development. Bon1 interacts with two receptor like protein kinases bir1 and BAK1, which may act on the same signaling pathway through the phosphorylation of bir1 and bon1 by BAK1, and cooperatively regulate temperature dependent plant growth and development and defense response (wanget al., 2011, plant J; yanget al, 2007,PlantPhysiol;Yangetal.,2006,PlantJ;YangandHua,2004,PlantCell)。

Lecture course

Plant physiology for undergraduates, advanced plant physiology and molecular biology for postgraduates and seminar for Botany

academic achievements

publish one’s thesis

DingY#,ShiY#,YangS*(2019)Advancesandchallengesinuncoveringcoldtoleranceregulato rymechanismsinplants.NewPhytol (invitedTansleyReview) Doi:10.1111/nph.15696. WangX,GuoC,PengJ,LiC,WanF,ZhangS,ZhouY,YanY,QiL,SunK,YangS,GongZ, LiJ*(2019)ABRE-BINDINGFACTORSplayaroleinthefeedbackregulationofABAsignalingbymediatingrapidABAinductionofABAco- receptorgenes.NewPhytol221 :341-355. FengY,LiuJ,ZhaiL,GanZ,ZhangG,YangS,WangY,WuT,ZhangX,XuX,HanZ*(2019)Naturalvariationincytokininmaintenanceimprovessalttolera nceinapplerootstocks.PlantCellEnviron42 :424-436. DingY,LvJ,ShiY,GaoJ,HuaJ, SongC,GongZ,YangS*(2019)EGR2phosphataseregulatesOST1kinaseactivityandfreezingto leranceinArabidopsis.EMBOJ38 :e99819.(SelectedforF1000Prime) ShiY#,DingY#,YangS*(2018)MolecularregulationofCBFsignal ingincoldacclimation.TrendsPlantSci (invitedreview)23:623-637. DingY,JiaY,ShiY,SongC,GongZ, YangS*(2018)OST1-mediatedBTF3LphosphorylationpositivelyregulatesCBFsduri ngplantcoldresponses.EMBOJ37 :e98228. LiuJ#,ShiY#,YangS*(2018)InsightsintotheregulationofC-repeatbindingfactors inplantcoldsignaling.JIntegrPlantBiol (invitedreview)60:780-795. HuangX,HouL,MengJ,YouH,LiZ,GongZ,Yan

Chinese PinYin : Yang Shu Hua