Chen Xiulan

Chen Xiulan

Since 1998, he has been engaged in the research of marine microorganism enzymology and marine biotechnology.

brief introduction

Female, born in August 1966, received a master of Science Degree in botany in 1992 and a doctor of Science Degree in microbiology in 2002. He was promoted to associate professor in 2002 and professor in 2004. In 2003, he was employed as a master's supervisor. Since 1998, he has been engaged in the research of marine microorganism enzymology and marine biotechnology.

Major teaching and research experience

Main teaching experience:

Undergraduate teaching

·Lecture: Plant Physiology and plant physiology experiment for undergraduates of Shandong University

·Lectures: plant tissue and cell culture and plant tissue and cell culture experiment

·General course of Shandong University: introduction to biotechnology

·Lecture: introduction to modern biotechnology, a required course for undergraduates majoring in bioengineering and a required course for undergraduates majoring in biotechnology, School of life, Shandong University.

Postgraduate teaching

1. From September 2003 to now, State Key Laboratory of microbial technology, Shandong University, master's supervisor,

From 2003 to 2013, a total of 4 master's students were recruited, including 2 graduates.

2. Assist professor Zhang Yuzhong to train 5 doctoral students and 10 master students.

3. For master's and doctoral students, we will give lectures on the elective course of "marine biotechnology".

Main research experience

·From September 1989 to July 1992, he completed his master's thesis in the Department of biology, Shandong Normal University. The main research content is the inhibition mechanism of salt stress on barley seed germination, tutor: Professor Zhao Kefu

·July 1992 to August 1998, cotton research center, Shandong Academy of Agricultural Sciences, research direction: Cultivation of new cotton varieties.

·From September 1998 to July 2002, he completed his doctoral dissertation in the State Key Laboratory of microbial technology of Shandong University. Research direction: cold adapted mechanism of deep sea cold adapted bacteria and cold adapted protease. Tutor: Professor Gao Peiji.

From September 1998 to now, he has been engaged in teaching and scientific research in the State Key Laboratory of microbial technology of Shandong University. Has been engaged in marine biotechnology research. During the 10th Five Year Plan period, he presided over and undertook two national "863" projects, one Shandong outstanding youth fund project and one Shandong natural science fund project. He was promoted to associate professor in 2002 and professor in 2004.

Main innovation achievements, innovation points and scientific significance in recent five years

Main research direction: marine biotechnology

(1) Marine microbial enzymology mainly studies the structure of enzymes in marine extreme environment and the mechanism of adapting to extreme environment.

(2) Research on enzyme engineering technology for high value development of marine biological resources

(3) Structure, function and evolution of photosynthetic pigment system in algae

Innovative achievements

At present, most of the protease preparations used are mesophilic protease. The optimum reaction temperature of the enzyme is 50 ~ 55 ℃. The energy consumption is high in the process of protein enzymatic hydrolysis. Considering the above shortcomings of mesophilic protease, a new tool enzyme, cold adapted protease, was developed. Because of its high catalytic efficiency at low temperature, cold adapted protease also has great potential in detergent and food preservation It has good application prospects (Chen Xiulan, Zhang Yuzhong, et al., industrial microbiology, 2001, Vol. 31 (1), 52-53). Marine samples, especially deep-sea samples, are good materials for studying cold adapted microorganisms, and the research of deep-sea microbiology is a hot spot in 2013 (Chen Xiulan, Zhang Yuzhong et al., marine science, 2004, 28 (1), 60-66; Chen Xiulan, Zhang Yuzhong et al., Chinese Journal of bioengineering, 2003, 23 (2), 86-90). Therefore, since 1999, our laboratory has carried out systematic research on the cold adapted protease produced by cold adapted microorganisms in deep-sea mud, and presided over and undertaken the National Marine 863 project, the National Natural Science Foundation project, as well as the Shandong Provincial doctor's fund, natural science foundation and scientific and technological research projects. The results are as follows

1) More than 200 strains of psychrotrophic bacteria producing psychrotrophic protease were isolated from 1850m deep sea mud in Okinawa Trough, Japan Pseudoaltermonassp.SM9913 The characteristics of enzyme production and cold adapted growth mechanism were studied, which provided strain materials for the study of marine cold adapted protease (Chen Xiulan, Zhang Yuzhong, et al., marine science, 2001,25 (1), 4-8; Chen Xiulan, Zhang Yuzhong, et al., high tech communication, 2002, 12 (5), 95-98).

2) Cold adapted bacteria from deep sea Pseudoaltermonassp.SM9913 MCP-01, a typical cold adapted protease, was isolated and purified from mesophilic protease. The optimum temperature of MCP-01 activity was 30 ~ 35 ℃, which was about 20 ℃ lower than mesophilic protease. The activity of MCP-01 could still maintain 12.2% at 0 ℃. It has a wide application prospect (Chen Xiulan, Zhang Yuzhong et al., marine science, 2001,25 (1), 4-8; Chen Xiulan, Zhang Yuzhong et al., marine biology, 2003, 989-993）。

3) Cold adapted bacteria were observed Pseudoaltermonassp.SM9913 ， Pseudomonassp.SM9915 In addition to the cold adapted enzymes, there are also the same kind of moderate temperature enzymes. A new idea that the same kind of enzymes are not synchronous evolutionary adaptation in the process of cold adapted evolution is proposed (Chen Xiulan, Zhang Yuzhong et al., ocean and Limnology, 2003, 34 (2), 155-160; Chen Xiulan, Zhang Yuzhong et al., marine biology, 2003, 989-993).

4) The autolysis process of MCP-01 was observed by capillary electrophoresis. It is suggested that the thermal sensitivity of MCP-01 is mainly due to the autolysis of enzyme. Therefore, the prevention of autolysis is the key problem in the application of cold adapted protease (Sun Caiyun, Chen Xiulan, Zhang Yuzhong, marine science, 2002, 26 (11), 71-73; Chen Xiulan, 2002)

Zhang Yuzhong et al., biotechnology letters, 200325:1763-1767). It was found that trehalose had a good protective effect on the stability of MCP-01, and the main mechanism was to prevent the autolysis of MCP-01 (Li Jianwei, Chen Xiulan, et al., Journal of Shandong University, 200338 (4): 116-119; Pan Jun, Chen Xiu LAN, zangyuzhong, protein and peptide letter, 2005, 12: At the same time, the protective effects of sucrose, mannose, galactose and glucose on the thermal stability of MCP-01 were discussed. Sucrose and galactose had better protective effects, while mannose and glucose had little effect (Chen Xiu LAN, Zhang Yu Zhong, J.of protein chemistry, 2006, P < 0.05), The results lay a foundation for further study on the interaction between sugar and protein. Several monosaccharides, disaccharides and crosslinking agents were used to modify MCP-01. The effects of modification on the stability of MCP-01 were studied by reaction kinetics, thermal inactivation kinetics, UV, fluorescence and circular dichroism (Lu Jingtao, Chen Xiulan, Zhang Yuzhong et al., J ofMolecular.Catalysis ,2006,submitted）。

5) It was found that the optimum pH, temperature, enzyme activity and thermal sensitivity of MCP-01 were different in four different buffer solutions of carbonic acid, phosphoric acid, Tris and boric acid. The results also showed that the autolysis rate and mode of MCP-01 were different in four buffer solutions of carbonic acid, phosphoric acid, Tris and boric acid, which provided a basis for studying the relationship between the structure and function of the cold adapted enzyme It provides important clues (Chen Xiulan, Zhang Yuzhong et al., J. of protein chemistry, 2002, P. R. China), The cold adapted and heat sensitive mechanism of MCP-01 were studied by CD, absorption and fluorescence spectroscopy combined with capillary electrophoresis. The results showed that the structure of MCP-01 was flexible and loose, and it was easy to be affected by ionic strength and charge in buffer solution, which was the main mechanism of cold adapted and heat sensitive, Eur.J.Biochem.2006,submitted）。

6) The gene of MCP-01 was cloned and sequenced. Blast analysis showed that MCP-01 and the other 10 genes in the database were analyzed from the genome sequence

Chinese PinYin : Chen Xiu Lan